Products/Cell Culture Media

Mesenchymal Stem Cell Media

Mesenchymal Stem Cell Media

Renaissance Mesenchymal Stem Cell Medium is a cell line derivation and maintenance medium specifically formulated for the extended in vitro propagation of mesenchymal stem cells derived from various tissue types.


Renaissance Mesenchymal Stem Cell Medium is a cell line derivation and maintenance medium specifically formulated for the extended in vitro propagation of mesenchymal stem cells derived from various tissue types.

Tissue Type: All


Storage and Stability

Store basal media at 4°C. Store supplement at -20°C. Media and supplement are stable for a minimum of 3 months from date of receipt, when stored as directed.

Quality Control

Renaissance Essential MSC Medium is tested for the ability to support proliferation and morphology during culture of mesenchymal stem cells over three continuous passages. Proliferation ability is measured as both cumulative popluation doublings and stability of growth rate. Product sterility is confirmed by negative test results for microbial contaminants including bacteria, fungi, and mycoplasma.

General Guidelines

Due to the variation in biology and quality of each tissue specimen, optimization of culture conditions is required for each new sample. These guidelines describe how to optimize the Renaissance Essential cell culture system for the derivation of cell lines from human tissue samples.

For additional support in the derivation of cell lines from primary human tumors using Renaissance Essential Medium, contact Cellaria Inc to learn about the training courses they provide (781-257-2679).

1. Use 0.4 mL of Renaissance™ Essential Tumor Medium per cm2 culture surface area.

Note: Limiting the culture medium volume will negatively impact culture health.

2. Test cell attachment on different culture surfaces for each new specimen.

3. Use medium color as an indicator to determine when to change the medium. Replace the medium when it becomes pink in order to replenish nutrients and maintain a stable pH.

Begin new cultures with a minimal amount of heat inactivated FBS (5%).

To make REM-MSC Complete Medium please combine the following:

  • 500 mL REM Basal Medium
  • 15 mL REM-MSC Supplement (15 mL, thawed briefly in a 37°C water bath)
  • Pen-strep or other antibiotics as desired


MSC PASSAGING PROTOCOL

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Regular price
$295.00
Sale price
$295.00
Regular price
Sold out
Unit price
per 

SIZE
Components (2):
500 ml MSC Basal Medium 
15 ml MSC Supplement

AVAILABILITY
In stock

PRODUCT CODE
CM-0201