Products/Cell Culture Media

T-Cell Media

T-Cell Media

Cellaria T-Cell Medium is a cell line derivation and maintenance medium specifically formulated for the extended in vitro propagation of T-cells derived from blood samples.


Cellaria Renaissance T-Cell Medium is a cell line derivation and maintenance medium specifically formulated for the extended in vitro propagation of T-cells derived from blood samples.

Tissue Type: Whole Blood


Storage and Stability

Store basal media at 4°C. Store supplement at -20°C. Media and supplement are stable for a minimum of 3 months from date of receipt, when stored as directed.

Quality Control

Renaissance Essential T-Cell Medium contains all the necessary growth factors to activate and expand T cells from PBMC's in combination with the CloudzTM T Cell Activation Kit. No additional growth factors or cytokines are required. Proliferation ability is measured as both cumulative population doublings and stability of growth rate. Product sterility is confirmed by negative test results for microbial contaminants including bacteria, fungi, and mycoplasma.

General Guidelines

Due to the variation in biology and quality of each tissue specimen, optimization of culture conditions is required for each new sample. These guidelines describe how to optimize the Renaissance Essential cell culture system for the derivation of cell lines from human tissue samples.

For additional support using Renaissance Essential Medium, contact Cellaria Inc to learn about the training courses they provide (781-257-2679).

1. Use 0.2 mL of Renaissance™ Essential Tumor Medium per cm2 culture surface area.

Note: Limiting the culture medium volume will negatively impact culture health.

2. Media changes should be performed every 3-4 days to replenish nutrients and maintain a stable pH.

To make REM-T Complete Medium please combine the following:

  • 500 mL REM Basal Medium
  • REM-T Supplement (thaw overnight @ 4°C or quickly @ 37°C)
  • 25 mL heat inactivated FBS (Hyclone SH3007103HI or equivalent) for final 5%.
  • 125 µL cholera toxin (100 µg/mL stock solution in cell culture grade water from EMD Millipore 227026-1 mg or equivalent) for final 25 ng/mL.
  • 5mL Pen/Strep (10,000 U/mL) for final 1%.


PRODUCT HANDLING AND DIRECTIONS FOR USE

T-Cell Activation SOP can be viewed and downloaded by scanning the above QR code.

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Regular price
$295.00
Sale price
$295.00
Regular price
Sold out
Unit price
per 

SIZE
Components (2):
500 ml REM-Basal Medium
15 ml T-Cell Supplement 

AVAILABILITY
In stock

PRODUCT CODE
CM-0301